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3 in the 1960s, after several years of extensive use, chloramphenicol s popularity began to diminish when toxicity was linked to two distinct effects on the bone marrow a a predictable dose-dependent toxicity resulting in anemia that was reversible upon drug withdrawal, and b an idiosyncratic reaction resulting in aplastic anemia that was unpredictable, irreversible, and frequently fatal, occurring with an incidence of 1 case in 24,000 to 40,000 courses of therapy.

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Of the two putative chloramphenicol resistance genes identified in the genome of bct-7112 t , btoyo_3723, and btoyo_4985, the later was chosen as the former appeared to have been truncated.

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